{"id":5418,"date":"2020-01-07T16:20:13","date_gmt":"2020-01-07T16:20:13","guid":{"rendered":"https:\/\/www.blopig.com\/blog\/?p=5418"},"modified":"2020-01-07T20:54:50","modified_gmt":"2020-01-07T20:54:50","slug":"b-cell-bispecificity","status":"publish","type":"post","link":"https:\/\/www.blopig.com\/blog\/2020\/01\/b-cell-bispecificity\/","title":{"rendered":"B-Cell Bispecificity?!"},"content":{"rendered":"\n<p>Happy New Year, Blopiggers!<br><br>Just a quick one from me this time around, to draw your attention to this intriguing paper by Shi <em>et al<\/em>., published in Nature Cell Discovery late last year.<br><br><strong>&#8220;<a href=\"https:\/\/www.nature.com\/articles\/s41421-019-0137-3\">More than one antibody of individual B cells revealed by single-cell immune profiling<\/a>&#8220;<\/strong><br><em>Zhan Shi, Qingyang Zhang, Huige Yan, et al.<br>Nature Cell Discovery (2019) 5:64<\/em><\/p>\n\n\n\n<p>Single-cell transcriptomics (e.g. using TenX sequencing) is beginning to yield fascinating insights into the inner workings of our immune system. It has long been thought that a single B cell can only express one antibody variable domain on its surface, accounted for by theories such as allelic exclusion and isotype exclusion.<\/p>\n\n\n\n<!--more-->\n\n\n\n<p>Shi <em>et al<\/em>. have detected that B cells commonly express an entirely different VH transcript to the one comprising its dominant B-Cell Receptor (5-10% of all VH transcripts). It doesn&#8217;t stop with the heavy chain either, as a whopping 60% of single B cells co-expressed Vkappa and Vlambda light chains.<\/p>\n\n\n\n<p>This points to an entirely new mechanism of induced repertoire diversity, that individual B cells can shuffle their V, D, and J transcripts after maturation to display different cell-surface receptors. It also hints at the possibility that natural B-cells could possess bispecificity (with two entirely different BCR binding sites simultaneously engaging one or more antigens) &#8211; a realisation that would embolden pharmaceutical companies in their efforts to engineer bispecific antibody therapeutics.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Happy New Year, Blopiggers! Just a quick one from me this time around, to draw your attention to this intriguing paper by Shi et al., published in Nature Cell Discovery late last year. &#8220;More than one antibody of individual B cells revealed by single-cell immune profiling&#8220;Zhan Shi, Qingyang Zhang, Huige Yan, et al.Nature Cell Discovery [&hellip;]<\/p>\n","protected":false},"author":45,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"nf_dc_page":"","wikipediapreview_detectlinks":true,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"ngg_post_thumbnail":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[186],"tags":[],"ppma_author":[532],"class_list":["post-5418","post","type-post","status-publish","format-standard","hentry","category-immunoinformatics"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"authors":[{"term_id":532,"user_id":45,"is_guest":0,"slug":"matt","display_name":"Matthew Raybould","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/71f525eb0d7861fad46de36f6b8f3a4cfd89c5cc6b13e48c5dc6e79a82c5b591?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/posts\/5418","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/users\/45"}],"replies":[{"embeddable":true,"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/comments?post=5418"}],"version-history":[{"count":5,"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/posts\/5418\/revisions"}],"predecessor-version":[{"id":5426,"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/posts\/5418\/revisions\/5426"}],"wp:attachment":[{"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/media?parent=5418"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/categories?post=5418"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/tags?post=5418"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/www.blopig.com\/blog\/wp-json\/wp\/v2\/ppma_author?post=5418"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}