Category Archives: Publications

[Publication] Effect of Single Amino Acid Substitution Observed in Cancer on Pim-1 Kinase Thermodynamic Stability and Structure

In this study we selected point mutations resulting in Pim-1 variants that are expressed in cancer tissues and reported in SNP databases, such as FastSNP and COSMIC. These Pim-1 variants have been comprehensively characterized to investigate the effect of single amino acid substitution on Pim-1 thermal and thermodynamic stability and structure in solution. Our results indicate that the effects of the mutation observed in cancer tissues cause local changes of tertiary structure, but do not affect binding to type I kinase inhibitors.

This work has been pioneered by researches at the Department of Biochemical Sciences “A. Rossi Fanelli”, Sapienza University of Rome and served as an inspiration for one of my thesis chapters.

[Publication] Memoir: template-based structure prediction for membrane proteins

Congratulations to all involved in the Memoir publication in Nucleic Acids Research! 

Memoir is a web server which builds homology models for membrane proteins.  It is a web-enabled workflow combining some of OPIG’s software; MP-T, IMembrane, Medeller & Fread.  The inputs are a sequence of the membrane protein you wish to model (target) and a PDB file to use as template.

Memoir may be found here and there is also a video tutorial narrated by Jamie.  There is even a funny blooper of him practising, which I kept to celebrate this moment.

Happy modelling!